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Validation of

Youfen Xu, Zong Li, Ruyin Liu, Hongxia Liang, Zhisheng Yu, Hongxun Zhang

《环境科学与工程前沿(英文)》 2020年 第14卷 第4期 doi: 10.1007/s11783-020-1246-9

摘要: Abstract • Pig feces is the predominant excrement produced by animal husbandry in China. • The PF, Pig-1-BacTaqMan, and Pig-2-BacTaqMan MST assays showed better performance. • The pig-specific MST assays can contribute to managing the pig fecal pollution. In China, pig feces is the predominant source of excrement produced by animal husbandry. Improper use or direct discharge of pig feces can result in contamination of natural water systems. Microbial source tracking (MST) technology can identify the sources of fecal pollution in environmental water, and contribute to the management of pig fecal pollution by local environmental protection agencies. However, the accuracy of such assays can be context-dependent, and they have not been comprehensively evaluated under Chinese conditions. We aimed to compare the performance of five previously reported pig-specific MST assays (PF, Pig-Bac1SYBR, Pig-Bac2SYBR, Pig-1-BacTaqMan, and Pig-2-BacTaqMan, which are based on Bacteroidales 16S rRNA gene markers) and apply them in two rivers of North China. We collected a total of 173 fecal samples from pigs, cows, goats, chickens, humans, and horses across China. The PF assay optimized in this study showed outstanding qualitative performance and achieved 100% specificity and sensitivity. However, the two SYBR green qPCR assays (Pig-Bac1SYBR and Pig-Bac2SYBR) cross-reacted with most non-pig fecal samples. In contrast, both the Pig-1-BacTaqMan and Pig-2-BacTaqMan assays gave 100% specificity and sensitivity. Of these, the Pig-2-BacTaqMan assay showed higher reproducibility. Our results regarding the specificity of these pig-specific MST assays differ from those reported in Thailand, Japan, and America. Using the PF and Pig-2-BacTaqMan assays, a field test comparing the levels of pig fecal pollution in rivers near a pig farm before and after comprehensive environmental pollution governance indicated that pig fecal pollution was effectively controlled at this location.

关键词: Microbial source tracking     Pig fecal pollution     16S rRNA gene markers     Pig-specific Bacteroidales    

EFFICIENT CONTAMINANT REMOVAL FROM LIQUID DIGESTATE OF PIG MANURE BY CHEMICAL PRECIPITATION AND CO MINERALIZATION

《农业科学与工程前沿(英文)》 2023年 第10卷 第3期   页码 479-491 doi: 10.15302/J-FASE-2023480

摘要:

● LFD was treated by fly ash-based chemical precipitation and CO2 mineralization.

关键词: anaerobic digestion     chemical oxygen demand     fly ash     ion removal     total phosphate    

EFFECT OF EXOGENOUS ADDITIVES ON HEAVY METAL PASSIVATION AND NITROGEN RETENTION IN PIG MANURE COMPOSTING

《农业科学与工程前沿(英文)》 doi: 10.15302/J-FASE-2023487

摘要:

● Research on heavy metal passivation and nitrogen emissions is necessary for the pig industry.

关键词: additives     composting     heavy metals passivation     nitrogen retention     pig manure    

AMMONIA DISPERSION FROM MULTI-FLOOR VERSUS STANDARD SINGLE-FLOOR PIG PRODUCTION FACILITIES BASED ON COMPUTATIONAL

《农业科学与工程前沿(英文)》 2023年 第10卷 第3期   页码 374-389 doi: 10.15302/J-FASE-2023501

摘要:

● NH3 dispersion from a multi-floor pig building was compared to a single-floor building.

关键词: pig building     computational fluid dynamics     ammonia     dispersion    

Measurement of trimethylamine concentration and evaluation of pig meat natural quality by a spectrophotometric

Mohammod Abdul HAMID,Xi WANG,Xiangdong DING,Chuduan WANG,Xingbo ZHAO

《农业科学与工程前沿(英文)》 2014年 第1卷 第3期   页码 236-241 doi: 10.15302/J-FASE-2014034

摘要: Pig meat off-flavor is attributed to trimethylamine (TMA) concentration, and it is considered as the precursor of the fishy off-flavor problems. In this study, TMA concentrations in pig meat were determined, and the interactions with breed and gender effects were discussed. In addition, the TMA threshold for meat off-flavor and pig meat natural quality was measured in relation to meat storage and movement, and the influential factors including the pig breed and storage time were discussed. The results indicated positive effects on the precursor of the fishy off-flavor and the TMA threshold. Native breeds were found to have lower TMA concentrations than European breeds ( <0.01), and females and castrated males had significantly lower TMA concentration than males ( <0.01), The threshold concentration of TMA when meat was classed as off-flavored was 25 μg·g , and this occurred after 35–38 h of storage. The natural qualities, such as appearance, flavor, color and overall acceptable scores declined significantly after 4 days in storage ( <0.01). It is concluded that pig meat off-flavor, breed and gender were essential factors affecting flavor for meat breeding programs, and storage time is important for pig meat natural quality.

关键词: pig meat     trimethylamine (TMA)     influential factor    

Reprogramming of the pig primordial germ cells into pluripotent stem cells: a brief review

Qijing LEI, Qin PAN, Shuai YU, Na LI, Shulin CHEN, Kuldip SIDHU, Jinlian HUA

《农业科学与工程前沿(英文)》 2019年 第6卷 第1期   页码 28-32 doi: 10.15302/J-FASE-2018222

摘要:

Primordial germ cells (PGCs) are regarded as unipotent cells that can produce only either spermatogonia or oocytes. However, PGCs can be converted into the pluripotent state by first dedifferentiation to embryonic germ cells and then by reprogramming to induce them to become pluripotent stem cells (iPSCs). These two stages can be completely implemented with mouse cells. However, authentic porcine iPSCs have not been established. Here, we discuss recent advances in the stem cell field for obtaining iPSCs from PGCs. This knowledge will provide some clues which will contribute to the regulation of reprogramming to pluripotency in farm species.

关键词: pig     pluripotent stem cells     primordial germ cells     reprogramming    

Genome-wide association study of the backfat thickness trait in two pig populations

Dandan ZHU,Xiaolei LIU,Rothschild MAX,Zhiwu ZHANG,Shuhong ZHAO,Bin FAN

《农业科学与工程前沿(英文)》 2014年 第1卷 第2期   页码 91-95 doi: 10.15302/J-FASE-2014005

摘要: Backfat thickness is a good predictor of carcass lean content, an economically important trait, and a main breeding target in pig improvement. In this study, the candidate genes and genomic regions associated with the tenth rib backfat thickness trait were identified in two independent pig populations, using a genome-wide association study of porcine 60K SNP genotype data applying the compressed mixed linear model (CMLM) statistical method. For each population, 30 most significant single-nucleotide polymorphisms (SNPs) were selected and SNP annotation implemented using Build 10.2. In the first population, 25 significant SNPs were distributed on seven chromosomes, and SNPs on SSC1 and SSC7 showed great significance for fat deposition. The most significant SNP (ALGA0006623) was located on SSC1, upstream of the gene. In the second population, 27 significant SNPs were recognized by annotation, and 12 SNPs on SSC12 were related to fat deposition. Two haplotype blocks, M1GA0016251-MARC0075799 and ALGA0065251-MARC0014203-M1GA0016298-ALGA0065308, were detected in significant regions where the and genes were identified as contributing to fat metabolism. The results indicated that genetic mechanism regulating backfat thickness is complex, and that genome-wide associations can be affected by populations with different genetic backgrounds.

关键词: backfat thickness     SNP chip     genome-wide association study     compressed mixed linear model     pig    

Detection of genomic signatures for pig hairlessness using high-density SNP data

Ying SU,Yi LONG,Xinjun LIAO,Huashui AI,Zhiyan ZHANG,Bin YANG,Shijun XIAO,Jianhong TANG,Wenshui XIN,Lusheng HUANG,Jun REN,Nengshui DING

《农业科学与工程前沿(英文)》 2014年 第1卷 第4期   页码 307-313 doi: 10.15302/J-FASE-2014039

摘要: Hair provides thermal regulation for mammals and protects the skin from wounds, bites and ultraviolet (UV) radiation, and is important in adaptation to volatile environments. Pigs in nature are divided into hairy and hairless, which provide a good model for deciphering the molecular mechanisms of hairlessness. We conducted a genomic scan for genetically differentiated regions between hairy and hairless pigs using 60K SNP data, with the aim to better understand the genetic basis for the hairless phenotype in pigs. A total of 38405 SNPs in 498 animals from 36 diverse breeds were used to detect genomic signatures for pig hairlessness by estimating between-population ( ) values. Seven diversifying signatures between Yucatan hairless pig and hairy pigs were identified on pig chromosomes (SSC) 1, 3, 7, 8, 10, 11 and 16, and the biological functions of two notable genes, and were revealed. When Mexican hairless pigs were contrasted with hairypigs, strong signatures were detected on SSC1 and SSC10, which harbor two functionally plausible genes, and . KEGG pathway analysis showed a subset of overrepresented genes involved in the T cell receptor signaling pathway, MAPK signaling pathway and the tight junction pathways. All of these pathways may be important in local adaptability of hairless pigs. The potential mechanisms underlying the hairless phenotype in pigs are reported for the first time. and are interesting candidate genes for the hairless phenotype in Yucatan hairless and Mexico hairless pigs, respectively. , , and as well as other genes involved in the MAPK and T cell receptor signaling pathways may be important in environmental adaption by improved tolerance to UV damage in hairless pigs. These findings improve our understanding of the genetic basis for inherited hairlessness in pigs.

关键词: hairlessness     pig     selective sweeping    

全球首例猪心脏移植人体手术

Sarah C. P. Williams

《工程(英文)》 2022年 第16卷 第9期   页码 6-8 doi: 10.1016/j.eng.2022.07.006

Health impacts of air pollution in China

《环境科学与工程前沿(英文)》 2021年 第15卷 第4期 doi: 10.1007/s11783-020-1367-1

摘要:

•Impacts of air pollution on various body systems health in China were highlighted.

关键词: Health impacts     Air pollution     China     Advances    

Environmental pollution and DNA methylation: carcinogenesis, clinical significance, and practical applications

null

《医学前沿(英文)》 2015年 第9卷 第3期   页码 261-274 doi: 10.1007/s11684-015-0406-y

摘要:

Environmental pollution is one of the main causes of human cancer. Exposures to environmental carcinogens result in genetic and epigenetic alterations which induce cell transformation. Epigenetic changes caused by environmental pollution play important roles in the development and progression of environmental pollution-related cancers. Studies on DNA methylation are among the earliest and most conducted epigenetic research linked to cancer. In this review, the roles of DNA methylation in carcinogenesis and their significance in clinical medicine were summarized, and the effects of environmental pollutants, particularly air pollutants, on DNA methylation were introduced. Furthermore, prospective applications of DNA methylation to environmental pollution detection and cancer prevention were discussed.

关键词: environmental pollution     DNA methylation     cancer     biomarker     diagnosis     therapy     prevention    

Investigating the impact of air pollution on AMI and COPD hospital admissions in the coastal city of

《环境科学与工程前沿(英文)》 2022年 第16卷 第5期 doi: 10.1007/s11783-021-1490-7

摘要:

• The impact of air pollution on AMI/COPD hospital admissions were examined.

关键词: AMI     COPD     Air pollution exposure     GAM    

Experimental study of pollution effect on the behavior of high voltage insulators under alternative current

Hani BENGUESMIA, Nassima M’ZIOU, Ahmed BOUBAKEUR

《能源前沿(英文)》 2021年 第15卷 第1期   页码 213-221 doi: 10.1007/s11708-017-0479-1

摘要: In this paper, the impact of the conductivity and the distribution of pollution on the behavior of the high voltage insulator cap and pin 1512L, artificially polluted is described. An experimental model in form of a disc is proposed. This experimental model reproduces the real model which is the 1512L insulator. Besides, a comparative study is presented. For this comparative study, different solutions are adopted to s`imulate the pollution (containing NaCl+ distilled water) that has different conductivities for a discontinuous distribution of the pollution on the insulator under an AC voltage. Furthermore, the influence of the pollution on the flashover voltage and the leakage current is studied. Finally, the behavior of real and experimental model of the insulator is investigated.

关键词: 1512L insulator     circular model     artificial pollution     flashover     leakage current     discontinuous pollution     high voltage    

A critical review of the emerging research on the detection and assessment of microplastics pollution

《环境科学与工程前沿(英文)》 2022年 第16卷 第10期 doi: 10.1007/s11783-022-1563-2

摘要:

● Coastal and marine regions are the most studied for microplastic pollution.

关键词: Plastic pollution     Waste management     Global south     Sustainability     Marine pollution    

System engineering for water pollution control at the watershed level in China

Wei MENG,

《环境科学与工程前沿(英文)》 2009年 第3卷 第4期   页码 443-452 doi: 10.1007/s11783-009-0037-0

摘要: The present water pollution situation at watershed level in China has been systematically analyzed. The causes of water pollution are attributed to the extensive economic developmental pattern, poor wastewater treatment, and a lack of nonpoint pollution control. The problems of water pollution control at watershed level include a lack of thought and approach, developmental delay in the environmental standard system, an inadequate monitoring ability, and an inefficient implementation of laws and regulations. From 2006 to 2020, water pollution control and governance will be a national key specific project of science and technology in China. The strategies of this project include establishing a water pollution control system at watershed level, orienting a healthy aquatic ecosystem, conducting risk management, and using comprehensive methods. The goal is to establish and complete a technological system of pollution control and management in three five-year phases. The main tasks are to develop common technologies, management systems, and mechanisms for lake eutrophication control, river pollution control, urban water environmental pollution control, potable water safety, and water environmental management. The bottlenecks of water pollution control and management in China could be systematically removed, and the demonstration of the system engineering approach will be conducted at selected key watersheds.

关键词: water pollution control     watershed     system engineering     sustainability     integrated watershed management     key specific project of science and technology    

标题 作者 时间 类型 操作

Validation of

Youfen Xu, Zong Li, Ruyin Liu, Hongxia Liang, Zhisheng Yu, Hongxun Zhang

期刊论文

EFFICIENT CONTAMINANT REMOVAL FROM LIQUID DIGESTATE OF PIG MANURE BY CHEMICAL PRECIPITATION AND CO MINERALIZATION

期刊论文

EFFECT OF EXOGENOUS ADDITIVES ON HEAVY METAL PASSIVATION AND NITROGEN RETENTION IN PIG MANURE COMPOSTING

期刊论文

AMMONIA DISPERSION FROM MULTI-FLOOR VERSUS STANDARD SINGLE-FLOOR PIG PRODUCTION FACILITIES BASED ON COMPUTATIONAL

期刊论文

Measurement of trimethylamine concentration and evaluation of pig meat natural quality by a spectrophotometric

Mohammod Abdul HAMID,Xi WANG,Xiangdong DING,Chuduan WANG,Xingbo ZHAO

期刊论文

Reprogramming of the pig primordial germ cells into pluripotent stem cells: a brief review

Qijing LEI, Qin PAN, Shuai YU, Na LI, Shulin CHEN, Kuldip SIDHU, Jinlian HUA

期刊论文

Genome-wide association study of the backfat thickness trait in two pig populations

Dandan ZHU,Xiaolei LIU,Rothschild MAX,Zhiwu ZHANG,Shuhong ZHAO,Bin FAN

期刊论文

Detection of genomic signatures for pig hairlessness using high-density SNP data

Ying SU,Yi LONG,Xinjun LIAO,Huashui AI,Zhiyan ZHANG,Bin YANG,Shijun XIAO,Jianhong TANG,Wenshui XIN,Lusheng HUANG,Jun REN,Nengshui DING

期刊论文

全球首例猪心脏移植人体手术

Sarah C. P. Williams

期刊论文

Health impacts of air pollution in China

期刊论文

Environmental pollution and DNA methylation: carcinogenesis, clinical significance, and practical applications

null

期刊论文

Investigating the impact of air pollution on AMI and COPD hospital admissions in the coastal city of

期刊论文

Experimental study of pollution effect on the behavior of high voltage insulators under alternative current

Hani BENGUESMIA, Nassima M’ZIOU, Ahmed BOUBAKEUR

期刊论文

A critical review of the emerging research on the detection and assessment of microplastics pollution

期刊论文

System engineering for water pollution control at the watershed level in China

Wei MENG,

期刊论文